Adrenergic Receptor Protocols by Curtis A. Machida

By Curtis A. Machida

Oregon overall healthiness Sciences Univ., Beaverton. offers a suite of reproducible equipment for the examine of regulators of CNS-mediated habit and neural functionality. For neurobiologists, neurochemists, neurologists, and pharmacologists. DNLM: Receptors, Adrenergic - research.

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Oligonucleotide synthesis facility. Sequences of adrenergic receptor gene subtypes. Computer programs for analyzing primers. Computer programs for comparing DNA sequences. Random hexamers, oligo dT17 primers or gene-specific antisense primers. Reverse transcriptase enzymes. PCR enzymes and reagents. Thermal cycler. 5. Cloning of PCR Products 1. 2. 3. 4. 5. Cloning vectors. TA cloning kits: TA Cloning or TOPO-Cloning kit (Invitrogen, Carlsbad, CA). Restriction endonucleases. Supplies for bacterial plating.

Delidow, B. , Lynch, J. , Peluso, J. , and White, B. A. (1996) Polymerase chain reaction: basic protocols, in Methods in Molecular Biology: Basic DNA and RNA Protocols, vol. 58 (Harwood, A. ), Humana, Totowa, NJ, pp. 275–292. , and Bonnet, J. (1990) cDNA library construction from small amounts of unfractionated RNA: Association of cDNA synthesis with polymerase chain reaction amplification. Anal. Biochem. 188, 422–426. 34 Scofield, Deupree, and Bylund 32. DeFrancesco, L. (1997) Don’t clone alone: A profile of cDNA libraries and kits.

J. Physiol. 153, 586–600. 4. Bylund, D. B. (1988) Subtypes of α 2-adrenoceptors: pharmacological and molecular biological evidence converge. Trends Pharmacol. Sci. 9, 356–361. 5. Bylund, D. , Eikenberg, D. , Hieble, J. , Langer, S. , Lefkowitz, R. , Minneman, K. , et al. (1994) IV. International Union of Pharmacology Nomenclature of Adrenoceptors. Pharmacol. Rev. 46, 121–136. 6. Johnson, R. D. and Minneman, K. P. (1987) Differentiation of α1-adrenergic receptors linked to phosphatidylinositol turnover and cyclic AMP accumulation in rat brain.

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